Frequently Asked Questions
ESR4: Detection and characterisation of micro- and nanoplastics in beverages and food.
Overview: The ESR at KTH will develop filter based sample preparation strategy to separate micro- and nanoplastics from water, beverage (beer) and foodstuff (salt and honey). Two parallel routes will be investigated, one approach will be a stand-alone sample preparation module using filters with various pore sizes, and a second approach will use centrifugal microfluidics for integrated sample preparation. In collaboration with ESR3, the first stand-alone approach will be integrated and/or combined with inertial microfluidics to deliver different sized plastics for downstream analysis.
ESR9: Multispectral imaging flow cytometry for assessment of microplastic particles
Overview: The ESR will develop and apply a multispectral imaging flow cytometry (mIFC) approach to assess microplastic particles in the size range between 2 and 40 µm based on their geometrical and colorimetric properties. ´Different affinities of plastic materials to dye molecules will be utilized to discriminate between microplastic materials. The number and size of microplastic particles will be detected as well to determine their content. Combined with appropriate separation and enrichment techniques mIFC can assess microplastics from various matrices such as seawater, sediments, food, drinks, plants and tissues.
ESR10: High throughput Raman spectroscopy for analysis of microplastic particles
Overview: The ESR will develop a high-throughput Raman microspectrometer with laser line excitation for analysis of microplastic particles on a substrate or in continuous flow. The laser excitation wavelength, spectrometer and detector will be selected that are most appropriate for Raman-based detection of coloured and non-coloured microplastics. Automation will include particle localization on the substrate before acquisition of Raman spectra. Acquisition of microplastic particles in a microfluidic chip will be synchronized with an image-based detection. A database of microplastics will be compiled and chemometric data analysis will classify the microplastic particles. Commercial databases will be tested as alternative approaches.